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J Endod：牙髓干细胞与2,3,5,4''''-Tetrahydroxystilbene-2-O-β-D-glucoside移植加速大鼠牙槽骨再生

2019-04-12 lishiting MedSci原创

DPSC再生
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尽管人牙髓干细胞(hDPSCs)促进骨再生的治疗潜力已得到证实，然而对于其临床应用的疗效还需要进一步评估和检验。这篇研究的目的是为了评估2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (THSG)对hDPSCs成骨分化的作用以及检测THSG增强的hDPSCs成骨潜力对大鼠牙槽骨缺损的疗效。

尽管人牙髓干细胞(hDPSCs)促进骨再生的治疗潜力已得到证实，然而对于其临床应用的疗效还需要进一步评估和检验。这篇研究的目的是为了评估2，3，5，4'-tetrahydroxystilbene-2-O-β-D-glucoside (THSG)对hDPSCs成骨分化的作用以及检测THSG增强的hDPSCs成骨潜力对大鼠牙槽骨缺损的疗效。研究通过实时定量聚合酶链式反应检测成骨相关mRNA表达(including ALP， RUNX2， BGLAP， and AMBN)。Alizarin red S染色观察分析THSG诱导的hDPSCs矿化。为了评估施加THSG的hDPSCs对牙槽骨再生的作用，研究构建雄性Sprague-Dawley大鼠牙槽骨缺损模型。缺损处分别施以Matrigel (Corning Inc， Corning， NY)， hDPSCs或hDPSCs + THSG处理。术后2周，通过micro-CT和组织学观察分析缺损的愈合情况。结果显示，在细胞模型中，THSG能够诱导成骨分化相关基因(ALP， RUNX2， and BGLAP)和釉质相关基因(AMBN)的表达。2周后，a

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    2019-05-08 bsmagic9140

    #TRA#

    0

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    2019-07-05 jxrzshh

    #牙髓#

    0

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    2019-09-26 mei540

    #牙槽骨#

    0

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    2020-01-17 yhy100200

    #TET#

    0

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    2019-11-05 一叶知秋

    #牙槽骨再生#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1657154, encodeId=7660165e15438, content=<a href='/topic/show?id=3ca31e71903' target=_blank style='color:#2F92EE;'>#TRA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17719, encryptionId=3ca31e71903, topicName=TRA)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=757b24868428, createdName=bsmagic9140, createdTime=Wed May 08 19:36:00 CST 2019, time=2019-05-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1669755, encodeId=2c6c1669e55d7, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=49, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Fri Jul 05 23:36:00 CST 2019, time=2019-07-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1667913, encodeId=5843166e913cf, content=<a href='/topic/show?id=64596e594d8' target=_blank style='color:#2F92EE;'>#牙槽骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=50, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67594, encryptionId=64596e594d8, topicName=牙槽骨)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=87b426195956, createdName=mei540, createdTime=Thu Sep 26 05:36:00 CST 2019, time=2019-09-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2011407, encodeId=ffc1201140e26, content=<a href='/topic/show?id=840d1e300e5' target=_blank style='color:#2F92EE;'>#TET#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17300, encryptionId=840d1e300e5, topicName=TET)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=cc52251, createdName=yhy100200, createdTime=Fri Jan 17 18:36:00 CST 2020, time=2020-01-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1957826, encodeId=1d9d195e82677, content=<a href='/topic/show?id=aaf96e596e0' target=_blank style='color:#2F92EE;'>#牙槽骨再生#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67596, encryptionId=aaf96e596e0, topicName=牙槽骨再生)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=30bd35, createdName=一叶知秋, createdTime=Tue Nov 05 23:36:00 CST 2019, time=2019-11-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1472184, encodeId=96c114e2184a9, content=<a href='/topic/show?id=d068101610b9' target=_blank style='color:#2F92EE;'>#骨再生#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101610, encryptionId=d068101610b9, topicName=骨再生)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a4cf7208806, createdName=lsj630, createdTime=Sun Apr 14 05:36:00 CST 2019, time=2019-04-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544902, encodeId=d312154490232, content=<a href='/topic/show?id=f5576e67147' target=_blank style='color:#2F92EE;'>#牙髓干细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=71, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67671, encryptionId=f5576e67147, topicName=牙髓干细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=841913671773, createdName=ms6519009802973839, createdTime=Sun Apr 14 05:36:00 CST 2019, time=2019-04-14, status=1, ipAttribution=)]
    2019-04-14 lsj630

    #骨再生#

    0

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    2019-04-14 ms6519009802973839

    #牙髓干细胞#

    0

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