笔记详情
标题
Gene array analysis
内容
Gene array analysis
Total RNA was isolated from passage 1 (young p1) and passage 5 (aged p5) MEFs with a RNeasy mini kit (Qiagen, Venlo, The Netherlands). During cDNA synthesis, samples were labeled with [33P]-deoxycytidine triphosphate (dCTP; MP Biochemicals, Irvine, CA). Mouse filter gene arrays (GF400a; Research Genetics, Invitrogen) were hybridized and analysis was performed as described previously.34 Expression patterns were verified by reverse transcription-polymerase chain reaction (RT-PCR).
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来源
Blood. 2006 March 1; 107(5): 2170–2179.
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