笔记详情
标题
In vitro differentiation of purified HSCs
内容
In vitro differentiation of purified HSCs
Purified Lin-Sca-1+c-kit+ (LSK) cells were cultured in IMDM (Invitrogen) containing 100 ng/mL PEG-rrSCF (Amgen) and 10 ng/mL GM-CSF (Behringwerke, Marburg, Germany) to stimulate rapid in vitro differentiation. Total cell number was assessed using trypan blue exclusion. At different time points during culturing RNA was isolated for gene expression analysis.
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来源
Blood. 2006 March 1; 107(5): 2170–2179.
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